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. 2022 Mar 18;298(5):101847. doi: 10.1016/j.jbc.2022.101847

Figure 3.

Figure 3

Contribution of TRPV4 channels to the PLC, PLA2, and CYP450 pathway-mediated colonic epithelial Cl-secretion.A and B, representative time courses and summary data showing the effect of TRPV4 activator RN1747 (40 μM) on CCh (50 μM)-stimulated distal colonic Isc (n = 5) in the absence or the presence of U73122 (30 μM) or U73343 (10 μM). C and D, representative time courses and summary data showing the effect of RN1747 on CCh-stimulated distal colonic Isc (n = 6) in the absence or the presence of MAFP (10 μM) or MAFP plus arachidonic acid (AA, 50 μM). E and F, representative time courses and summary data showing the effect of RN1747 on CCh-stimulated distal colonic Isc (n = 5) in the absence or the presence of miconazole (Mic, 10 μM) or Mic plus AA. Ctrl represents as the control with CCh treatment only. CCh adds to serosal side. RN1747, U73122, U73343, MAFP, AA, and Mic add to both sides. The data are presented as mean ± SD. ∗p < 0.05 was performed by Student’s t test. ns, no significant differences. CCh, carbachol; Isc, short-circuit current; MAFP, methyl arachidonyl fluorophosphonate; PLA, phospholipid A; PLC, phospholipid C; TRPV, transient receptor potential vanilloid.