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. 2022 Mar 23;298(5):101850. doi: 10.1016/j.jbc.2022.101850

Figure 1.

Figure 1

EV71 infection induces pyroptosis.A and B, HeLa cells were mock infected or infected with EV71 at various MOIs as indicated. At 24 h after infection, LDH release–based cell death (A) and ATP cell viability were assessed. C, EV71 replication in HeLa cells. After infection, HeLa cells were fixed and stained with an antibody against EV71 (green). Nuclei were counterstained with DAPI (blue). The scale bar represents 100 μm. D, pyroptosis induced by EV71 infection. HeLa cells were mock infected or infected with EV71. At the indicated time points, phase-contrast imaging was performed. Images were obtained by microscopy. The scale bar represents 50 μm. All data are mean values ± SD. Experiments were performed as triplicates and repeated at least twice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. DAPI, 4',6-diamidino-2-phenylindole; EV71, enterovirus 71; LDH, lactate dehydrogenase; MOI, multiplicity of infection.