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. 2022 Mar 23;298(5):101850. doi: 10.1016/j.jbc.2022.101850

Figure 2.

Figure 2

EV71 infection induces the cleavage of GSDME.A and B, HeLa (A) and SK-N-SH (B) cells were mock infected or infected with EV71 at MOIs of 2 or 0.2, respectively. At the indicated time points, cell lysates were analyzed by Western blotting with antibodies against GSDME, which recognized the N-terminal region of GSDME, EV71, and β-actin. C and D, HeLa (C) and SK-N-SH (D) cells were infected with EV71 at various MOIs as indicated for 24 h. Cell lysates were analyzed by Western blotting as described in (A) and (B). E and F, RD (E) and 293T (F) cells were mock infected or infected with EV71. At the indicated time points, cell lysates were analyzed as described in (A) and (B). Data are representative of three independent experiments. EV71, enterovirus 71; GSDME, gasdermin E; MOI, multiplicity of infection; RD, rhabdomyosarcoma.