TABLE 6.
Items | Groups1 |
SEM | P-value | ||||
H (33.0%) | MH (29.9%) | M (28.1%) | ML (26.5%) | L (24.8%) | |||
Phylum (%) | |||||||
Bacteroidetes | 53.713a | 55.471a | 57.010a | 54.551a | 42.073b | 1.224 | < 0.001 |
Firmicutes | 43.572b | 41.801b | 39.772b | 42.100b | 52.510a | 1.321 | 0.019 |
Tenericutes | 0.779b | 0.789b | 1.173b | 1.275b | 1.821a | 0.154 | < 0.001 |
Spirochetes | 0.470b | 0.621b | 0.887ab | 0.763b | 1.259a | 0.136 | 0.002 |
Planctomycetes | 0.680b | 0.355b | 0.414b | 0.421b | 1.157a | 0.117 | 0.033 |
Genus (%) | |||||||
Prevotella 1 | 26.776b | 29.889a | 22.167c | 20.669c | 15.518d | 0.696 | < 0.001 |
Rikenellaceae RC9 gut group | 5.571b | 4.105c | 7.484a | 7.905a | 6.117b | 0.276 | < 0.001 |
Prevotellaceae NK3B31 group | 1.482c | 1.795c | 2.347c | 4.510a | 3.468b | 0.291 | < 0.001 |
Prevotellaceae UCG-001 | 2.449a | 2.225a | 1.992b | 2.146ab | 1.910b | 0.206 | 0.037 |
Prevotellaceae UCG-003 | 0.881b | 1.479a | 1.505a | 1.426a | 0.937b | 0.084 | < 0.001 |
Ruminococcus 1 | 0.651b | 1.308a | 0.920b | 0.917b | 0.631b | 0.106 | 0.001 |
Ruminococcus 2 | 1.091bc | 0.812c | 1.131bc | 1.236b | 2.209a | 0.108 | 0.003 |
Ruminococcaceae UCG-002 | 0.230b | 0.427a | 0.345ab | 0.437a | 0.342ab | 0.086 | 0.022 |
Ruminococcaceae UCG-004 | 0.289ab | 0.197ab | 0.141b | 0.194ab | 0.306a | 0.046 | 0.022 |
Ruminococcaceae UCG-005 | 0.513b | 0.457b | 0.529b | 1.440a | 0.306b | 0.070 | < 0.001 |
Ruminococcaceae UCG-010 | 0.569ab | 0.638ab | 0.818a | 0.746ab | 0.450b | 0.099 | 0.029 |
Ruminococcaceae UCG-011 | 1.335b | 1.167b | 1.897a | 1.831a | 1.828a | 0.113 | < 0.001 |
Ruminococcaceae UCG-014 | 0.559c | 1.052b | 0.894bc | 0.703bc | 1.538a | 0.116 | < 0.001 |
Ruminococcaceae NK4A214 group | 11.212ab | 10.255bc | 8.576c | 10.476bc | 12.957a | 0.674 | 0.001 |
Lachnospiraceae AC2044 group | 0.519c | 3.990a | 0.861bc | 0.914b | 0.786bc | 0.108 | < 0.001 |
Lachnospiraceae UCG-004 | 0.424bc | 0.486b | 0.293c | 0.299c | 1.206a | 0.055 | < 0.001 |
Lachnospiraceae UCG-008 | 0.615b | 0.631b | 0.319c | 0.440bc | 0.927a | 0.124 | < 0.001 |
Christensenellaceae R-7 group | 13.871a | 7.129c | 7.240c | 10.002b | 14.791a | 0.608 | < 0.001 |
Butyrivibrio 2 | 0.789b | 1.009b | 1.969a | 1.049b | 0.976b | 0.144 | 0.049 |
Moryella | 0.595a | 0.273b | 0.309b | 0.273b | 0.437ab | 0.072 | 0.005 |
Pseudobutyrivibrio | 0.171b | 0.247b | 1.788a | 0.342b | 0.174b | 0.103 | < 0.001 |
Sporolactobacillus | 1.022ab | 0.874b | 0.878b | 0.904b | 1.512a | 0.156 | 0.010 |
Treponema 2 | 0.595a | 0.273b | 0.309b | 0.273b | 0.437ab | 0.072 | 0.005 |
p-1088-a5 gut group | 0.289ab | 0.197ab | 0.141c | 0.194ab | 0.306a | 0.046 | 0.036 |
Uncultured (%) | |||||||
f_F082| g_uncultured | 10.111a | 6.853c | 10.397a | 8.507b | 7.774bc | 0.284 | < 0.001 |
f_Muribaculaceae| g_uncultured | 3.389b | 5.085a | 6.045a | 5.877a | 3.100b | 0.292 | 0.015 |
f_Bacteroidales RF16 group| g_uncultured | 0.483bc | 0.772a | 0.667ab | 0.546b | 0.306c | 0.057 | < 0.001 |
f_Bacteroidales BS11 gut group| g_uncultured | 0.243b | 0.782a | 0.713a | 0.332b | 0.358b | 0.065 | < 0.001 |
f_Paludibacteraceae| g_uncultured | 0.365b | 0.427ab | 0.546a | 0.460ab | 0.398ab | 0.046 | 0.034 |
f_p-251-o5| g_uncultured | 0.463ab | 0.197c | 0.634a | 0.289bc | 0.424b | 0.055 | < 0.001 |
f_Lachnospiraceae| g_uncultured | 0.930b | 1.134ab | 1.292a | 0.832b | 1.134ab | 0.093 | 0.004 |
o_Mollicutes RF39| f_uncultured | 0.772b | 0.782b | 1.150b | 1.256b | 1.814a | 0.153 | < 0.001 |
a–cMeans within a row with different superscripts differ significantly (p < 0.05).
1Groups: different peNDF1.18 (PS > 1.18 mm) contents of 33.0, 29.9, 28.1, 26.5, and 24.8% were obtained by chopping or crusher crushing the forage into the following lengths: H (7 cm), MH (4 cm), M (1 cm), ML (5–0 mm sieve), and L (1-mm sieve).