Internal standard mixture
| Reagent | Final concentration (in 100 μL sample) | Amount |
|---|---|---|
| Lactic acid-D3 (200 μM) | 10 μM | 5 μL |
| 13C3-Pyruvate (100 μM) | 5 μM | 5 μL |
| 13C6-D-Glucose (2,000 μM) | 100 μM | 5 μL |
| 13C6-D-Glucose-6-phosphate (200 μM) | 10 μM | 5 μL |
| 13C6-D-Fructose-1,6-diphosphate (200 μM) | 10 μM | 5 μL |
| Adenosine-15N5-triphosphate (1,000 μM) | 50 μM | 5 μL |
| Guanosine-15N5-triphosphate (1,000 μM) | 50 μM | 5 μL |
| Adenosine-15N5-monophosphate (1,000 μM) | 50 μM | 5 μL |
| Guanosine-15N5-monophosphate (1,000 μM) | 50 μM | 5 μL |
| Succinic acid-D6 (100 μM) | 5 μM | 5 μL |
| Citric acid-D4 (100 μM) | 5 μM | 5 μL |
| L-Carnitine-D3 (100 μM) | 5 μM | 5 μL |
| Thymine-D4 (200 μM) | 10 μM | 5 μL |
| 13C2-Glycine (10,000 μM) | 500 μM | 5 μL |
| Alanine-D4 (250 μM) | 12.5 μM | 5 μL |
| Arginine-D7 (250 μM) | 12.5 μM | 5 μL |
| Aspartate-D3 (250 μM) | 12.5 μM | 5 μL |
| 13C1-Citrulline (250 μM) | 12.5 μM | 5 μL |
| Glutamate-D3 (250 μM) | 12.5 μM | 5 μL |
| Glutamine-D5 (250 μM) | 12.5 μM | 5 μL |
| 13C6-Isoleucine (250 μM) | 12.5 μM | 5 μL |
| Leucine-D3 (250 μM) | 12.5 μM | 5 μL |
| Lysine-D4 (250 μM) | 12.5 μM | 5 μL |
| Methionine-D3 (250 μM) | 12.5 μM | 5 μL |
| Ornithine-D6 (250 μM) | 12.5 μM | 5 μL |
| Phenylalanine-D5 (250 μM) | 12.5 μM | 5 μL |
| Proline-D7 (250 μM) | 12.5 μM | 5 μL |
| Serine-D3 (250 μM) | 12.5 μM | 5 μL |
| Tryptophan-D5 (250 μM) | 12.5 μM | 5 μL |
| Tyrosine-D4 (250 μM) | 12.5 μM | 5 μL |
| Valine-D8 (250 μM) | 12.5 μM | 5 μL |
| Total volume of internal standard to be added to each sample | n/a | 155 μL |
Store this solution at −20°C.