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. 2022 Apr 11;9:857875. doi: 10.3389/fmolb.2022.857875

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Copyright © 2022 Wang, Xia, Zhu, Zeng, Wei, Lu, Li, Shi, Liu, Zhang and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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5′tsRNA-Gly-GCC targets 3′UTR of Sirt6 mRNA. (A) An illustration of the putative site of 5′tsRNA-Gly-GCC binding within the 3′UTR of Sirt6 predicted by bioinformatics software RNAhybrid, with green used to highlight those nucleotides mutated for a mutant version of this sequence. (B) Luciferase activity assay revealed that 5′tsRNA-Gly-GCC mimic transfection into Hepa1-6 cells resulted in a 40% reduction in luciferase activity for constructs containing a WT but not a mutated version of this 3′UTR of Sirt6 binding site (n = 7). (C) Luciferase activity assay revealed that 5′tsRNA-Gly-GCC inhibitor transfection into Hepa1-6 cells significantly increased WT Sirt6 3′UTR activity, while 5′tsRNA-Gly-GCC inhibitor also had no significant effect on MUT Sirt6 3′UTR (n = 7). ****p < 0.0001.