FIGURE 6.

FoxO1 activation caused by reduced FoxO1 nuclear exclusion and ubiquitination degradation in HFD-F1 liver. (A–D) Cytoplasmic and nuclear FoxO1 in liver tissue from HFD-F1 mice and CD-F1 mice was examined by Western blotting. Cytoplasmic protein β-actin and nuclear protein histone3 served as controls. (n = 4). (E) Liver tissue from HFD-F1 mice and CD-F1 mice were collected for the immunoprecipitation with FoxO1 antibody. FoxO1 and ubiquitinated FoxO1 protein were examined by Western blotting. (F) Schematic diagram of the mechanism by which 5′tsRNA-Gly-GCC regulates the Sirt6-FoxO1 pathway involved in enhanced gluconeogenesis in the offspring as a result of the paternal high-fat diet. ***p < 0.001.