Figure 2.

Expression profiles of clock genes in GEFs. Total RNA samples were collected at the indicated times following DXM synchronization. The mRNA expression levels of the clock genes (BMAL1, CLOCK, NPAS2, NR1D1, RORα, PER1, PER2, PER3, CRY1, and CRY2) and clock-controlled genes (DBP and E4BP4) were normalized to the housekeeping gene 36B4 and expressed as relative to the nadir. Red dots represent the relative values of each independent determination at the respective time points (n = 3). Cosinor analysis was utilized to determine the rhythmic expression patterns of the examined genes. The black solid lines show the fitted curves. P < 0.05 was considered to indicate rhythmic expression. GEFs, goat embryonic fibroblasts.