Figure 5.

mRNA and protein expression profiles of clock genes in male goat kidney tissues. The male goat kidney tissues were collected at two representative time points (10:00 h and 22:00 h). (A) The expression levels of BMAL1 and NR1D1 were examined by western blotting analysis. Representative blots and quantitative analysis from three independent experiments are shown. (B) The mRNA expression levels of clock genes (BMAL1, CLOCK, NPAS2, NR1D1, RORα, PER1, PER2, PER3, CRY1, and CRY2) and clock-controlled genes (DBP and E4BP4) were examined by qPCR. All data are presented as means ± SE (n = 3). Student's t-test was used to analyze the difference within different groups. Differences were considered significant at P < 0.05. Asterisks indicate significant differences. *P < 0.05, **P < 0.01, ***P < 0.001.