Table 3.
Cloning of a comprehensive set of clock genes and circadian gene promoters in the goat.
| Size (bp) | GenBank accession | Protein identity (%) | |||
|---|---|---|---|---|---|
| Cow | Mouse | Human | |||
| Expression vectors | |||||
| BMAL1 | 1881 | XM_018059578.1 | 99 | 96 | 98 |
| CLOCK | 2538 | XM_018049467.1 | 99 | 93 | 97 |
| CRY2 | 1791 | XM_018059193.1 | 93 | 93 | 95 |
| RORα | 1407 | NM_001285652.1 | 99 | 99 | 98 |
| NR1D1 | 1851 | XM_018065020.1 | 99 | 93 | 94 |
| Gene promoter reporters | |||||
| BMAL1 | 862 | NC_030822.1 | |||
| NR1D1 | 1657 | NC_030826.1 | |||
The complete coding sequence of five clock genes was cloned and used to construct the expression vectors. Proximal gene promoter regions of the Capra hircus BMAL1 and NR1D1 were also obtained. The table provides the size, GenBank accession number, and protein homology when applicable.