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. 2022 Feb 20;9(12):2200173. doi: 10.1002/advs.202200173

Figure 2.

Figure 2

The in vitro cytotoxicity and cellular uptake of micelles. a) The in vitro cytotoxicity of DOX‐loaded micelles against adherent BxPC‐3 and MCF‐7/ADR cells determined by the MTT assay (48 h treatment). b) The in vitro cytotoxicity of DOX‐loaded micelles against multicellular tumor spheroids (MTSs) determined by the resazurin assay (48 h treatment). c) Confocal images of MCF‐7/ADR cells cultured with the PEG‐Cy5PCL, OPDMA‐Cy5PCL, and OPDEA‐Cy5PCL micelles at Cy5‐eq. dose of 0.5 µg mL−1 for 1 h. Cell nuclei stained with Hoechst 33 342 are shown in blue, and Cy5‐labeled micelles are shown in red. Scale bar: 25 µm. d) Quantification of Cy5 intensity in the confocal images using ImageJ. e) Time‐dependent cellular uptake of the PEG‐Cy5PCL, OPDMA‐Cy5PCL, and OPDEA‐Cy5PCL micelles in MCF‐7/ADR cells measured by flow cytometry. Cy5‐eq. dose: 0.5 µg mL−1.