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. 2022 Feb 20;9(12):2200173. doi: 10.1002/advs.202200173

Figure 3.

Figure 3

Intracellular trafficking of Cy5‐labeled micelles in MCF‐7/ADR cells. a) Colocalization of PEG‐Cy5PCL, OPDMA‐Cy5PCL, and OPDEA‐Cy5PCL with mitochondria after 2‐h incubation. Scale bar: 25 µm. b) Overlapping profiles of Cy5 fluorescence with MitoTracker Green fluorescence along the selected line across the cell (indicated by a red line in the zoomed‐in image). c) Manders’ correlation coefficients of Cy5 and MitoTracker Green calculated by pixel intensity using ImageJ. Cells were cultured with PEG‐Cy5PCL, OPDMA‐Cy5PCL, and OPDEA‐Cy5PCL (Cy5‐eq. dose: 0.1 µg mL−1) for 2 h. The Cy5 fluorescence was shown in red, and MitoTracker Green was in green. Scale bar: 25 µm. d) The JC‐1 assay of the ΔΨm in MCF‐7/ADR cells. The cells were incubated with DOX formulations at a DOX‐eq. dose of 0.5 µg mL−1 or CCCP (1 mM) for 2 h. e) Intracellular ATP levels of MCF‐7/ADR cells exposed to DOX formulations at a DOX‐eq. dose of 0.5 µg mL−1 or CCCP (1 × 10−3 m) for 12 h.