Table 1.

Overview of common single‐cell protein analysis methods

Technique	Sample	Detection method	Multiplicity	Throughput	Sensitivity	Ref.
Single‐cell barcode chip (SCBC)	Single‐cell lysis	Fluorescence detection	About 40	Medium	High	[ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]
Miniaturized ELISA	Single‐cell	Fluorescence detection	About 30	Medium	Medium	[ 37 , 38 , 39 ]
Droplet‐based microfluidics methods	Single‐cell	Fluorescence detection	About 10	Medium	Medium	[ 40 , 41 , 42 , 43 , 44 , 45 , 46 ]
CE‐LIF	Single‐cell	Fluorescence detection	About 10	Medium	Medium	[ 47 , 48 ]
Single‐cell Western blotting (scWB)	Single‐cell lysis	Fluorescence detection	About 10	Medium	Medium	[ 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 ]
Proximity ligation assay‐based method	Single‐cell	RNA sequencing	Unlimited	Medium	High	[ 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 ]
Micro‐flow cytometry	Single cells	Fluorescence detection	Up to 30	Medium	Medium	[ 101 , 102 , 103 ]
Mass cytometry	Single cells	MS detection	Up to 50	High	Medium	[ 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 ]
Imaging mass cytometry	Fixed cell or tissue slides	MS detection	Up to 50	Medium	Medium	[ 107 ]
CITE‐Seq and REAP‐seq	Single‐cell	RNA sequencing	Unlimited	High	High	[ 117 , 118 , 119 ]
In situ protein analysis approach with cleavable fluorescent antibodies	Single cells	Fluorescence detection	Up to 100	Medium	High	[ 121 , 125 , 128 ]
Flow‐FISH	Single cells	Fluorescence detection	Up to 50	Medium	Medium	[ 178 , 179 , 180 ]
Pi‐ATAC	Single cells	RNA sequencing	Unlimited	High	High	[ 183 ]
NanoPOTS‐MS	Single cells	MS detection	About 1000	Medium	Medium	[ 141 , 142 , 147 ]
Nano‐LC‐MS	Single cells	MS detection	About 500	Medium	Medium	[ 144 , 145 ]
SCoPE‐MS	Single cells	MS detection	About 1000	Medium	Medium	[ 151 , 163 ]
CE‐MS	Single‐cell	MS detection	About 1000	Medium	Medium	[ 143 , 168 ]