Figure 3.

HTR1A inhibits the TGF‐β/TβRII/Smad canonical pathway. a,b) RNA‐seq of HTR1A overexpressing cells were analyzed by KEGG and GSEA, while TGF‐β and EMT pathway were enriched. c,d) HTR1A significantly inhibited the expression of TβRII and p‐Smad3 proteins, while no significant change was shown in the mRNA level. e–g) TGF‐β1 and LY2109761 rescued the signaling pathway and migration ability of breast cancer cells mediated by HTR1A. h) F‐actin staining of HTR1A overexpressing and knockdown cells. i) Cell adhesion analyzes of HTR1A overexpressing and knockdown cells. Data are means ± SD, n = 3. *p < 0.05 and **p < 0.01.