Figure 2.

MPEG1-1 observably enhanced PTC cell proliferation, migration, and invasion

(A) CCK-8 assay was adopted to analyze the effects of MPEG1-1 overexpression or knockdown on the proliferation of TPC1 or BCPAP cells at the appointed time. (B) Clony formation assay was used to evaluate the colony-forming capacity of MPEG1-1-overexpressed TPC1 cells and MPEG1-1-silenced BCPAP cells. (C) After MPEG1-1 overexpression or knockdown, cell proliferation was tested using EdU staining in TPC1 and BCPAP cells. (D) Wound healing displayed the change in cell migration in MPEG1-1-upregulated TPC1 cells and MPEG1-1-downregulated BCPAP cells. (E) Cell invasion was evaluated via Transwell assay in TPC1 or BCPAP cells, which were transfected with MPEG1-1-overexpressed plasmid or shRNAs. ∗p < 0.05, ∗∗∗p < 0.001.