Figure 5.

Cancer progression mediated by MPEG1-1 was partially reversed by miR-766-5p in PTC cells

TPC1 cells were co-transfected with MPEG1-1-overexpressed plasmid and miR-766-5p mimics, and BCPAP cells were co-transfected with MPEG1-1 shRNAs and miR-766-5p inhibitor. (A) Change of cell proliferation was assessed by CCK-8, ∗p < 0.05, ∗∗p < 0.01 versus vector group; ###p < 0.001 vs. MPEG1-1+NC group. (B) Colony formation assay was performed to evaluate the change in cell viability. (C) Change of cell viability was also analyzed via EdU staining. (D) Wound healing was performed to confirm the change in migration ability. (E) Transwell assay showed the change in cell invasion ability. ∗p < 0.05, ∗∗∗p < 0.001.