Figure 8.

SALI. In response to lysosome inhibition or impaired autophagosome maturation, autophagic intermediates containing autophagy cargo receptor proteins are diverted to late endosomes. Within these autophagosome–late endosome hybrid organelles, termed amphisomes, autophagy cargo receptors intermix with intraluminal vesicles that are formed through intraluminal budding mechanisms. The contents of amphisomes are subsequently released as EVPs via Rab27a-dependent exocytosis at the plasma membrane.