Figure 3.

NEAT1 binds to miR-122-5p and downregulates its expression. (a) Differential analysis of miRNA expression in OA-related GSE143514 microarray. (b) Venn diagram of the predicted downstream miRNAs of NEAT1 by the starBase database and the upregulated miRNAs in OA samples in the OA-related GSE143514 microarray. (c) Expression of miR-122-5p in OA samples in the OA-related GSE143514 microarray. (d) Binding sites between NEAT1 and miR-122-5p. (e) Luciferase activity of NEAT1-WT and NEAT1-MUT detected by dual-luciferase reporter assay. (f) Colocalization of Cy3-miR-122-5p (red) and FITC-NEAT1 (green) determined by FISH (scale bar = 25 μm). Nuclei were stained in blue by DAPI. (g) Interaction between NEAT1 and miR-122-5p detected by RIP. (h) Interaction between NEAT1 and miR-122-5p determined by RNA pull-down. (i) NEAT1 and miR-122-5p expression in cartilage tissues of OA patients (n = 25) and normal tissues (n = 25) (left) determined by RT-qPCR as well as the correlation analysis of NEAT1 expression and miR-122-5p expression in cartilage tissues of OA patients (n = 25) (right) by Pearson's correlation coefficient. ∗p < 0.05. Measurement data were expressed as mean ± standard deviation. Data between groups were compared using independent sample t test. Correlation was analyzed by Pearson's correlation analysis. The cell experiment was run in triplicate independently.