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. Author manuscript; available in PMC: 2022 Apr 25.
Published in final edited form as: Vaccine. 2008 Jul 25;26(37):4819–4829. doi: 10.1016/j.vaccine.2008.06.103

Fig. 2. Plasmid based mGM-CSF has a broad effective dosage range.

Fig. 2.

Balb/C mice (n=8/group) were immunized (i.m.) a single time with empty vector, with 100 mcg env pDNA alone or with env pDNA mixed with the indicated amount of pmGM-CSF or pmFlt-3L, or 25 mcg pmGM-CSF and pmFlt-3L in combination. Ten days post-immunization, splenocytes were isolated and env gp160-specific IFN-γ ELISpot responses were measured following stimulation with pooled env peptides (SFC, spot-forming cells). Data are reported as the mean value of each group with standard error of the mean indicated. * indicates mean responses significantly (p < 0.05) different from those obtained with the env pDNA vaccine alone.