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. 2022 Apr 22;16:11795549221092218. doi: 10.1177/11795549221092218

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© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 7. — The experimental verification of LINC00657 in the co-culture system. (A) The apoptosis assay of the NC group (HCT116). (B) The apoptosis assay of the sh-LINC00657 group (HCT116). (C) The apoptosis assay of the NC group (CD8+T cell). (D) The apoptosis assay of the sh-LINC00657 group (CD8+ T cell). (E) The colony formation assay of the NC and sh-LINC00657 group. (F) Histogram of clone formation assay. (G-I) The mRNA expression of GZMB, PRF1, and IFNG in sh-LINC00657 and NC group (CD8+ T cells). (J) The mRNA expression of CD155 in sh-LINC00657 and NC group (HCT116). (K) The expression of PRF1 and GZMB in CD8+ T cells by flow cytometry (NC group). (L) The expression of PRF1 and GZMB in CD8+ T cells by flow cytometry (sh-LINC00657 group).*P < 0.05, **P < 0.01, ***P < 0.001.