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. 2022 Apr 15;3(2):101316. doi: 10.1016/j.xpro.2022.101316

MST-buffer (Replace OG with whatever detergent and concentration are most suitable for the protein of interest)

Reagent Final concentration Amount
1 M Tris pH 8.0 20 mM 1 mL
5 M NaCl 300 mM 3 mL
OG 1% (w/v) 0.5 g
De-ionized H2O up to 50 mL
Total 50 mL

Store buffer with the added detergent at 4°C for a maximum of 3 days.