MST-buffer (Replace OG with whatever detergent and concentration are most suitable for the protein of interest)
Reagent | Final concentration | Amount |
---|---|---|
1 M Tris pH 8.0 | 20 mM | 1 mL |
5 M NaCl | 300 mM | 3 mL |
OG | 1% (w/v) | 0.5 g |
De-ionized H2O | up to 50 mL | |
Total | 50 mL |
Store buffer with the added detergent at 4°C for a maximum of 3 days.