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. 2001 Mar;45(3):727–733. doi: 10.1128/AAC.45.3.727-733.2001

TABLE 1.

Oligonucleotide primers

Primer Sequence (5′→3′) Application
23SP1 ACGGCGGCCGTAACTATA PCR of a 307-bp fragment of 23S rRNA gene; sequencing of the PCR fragment from both directions
23SP2 ACAGGCCAGTTAGCTA
gyrAP1 ATGCATGAATTAGGCCTTACT PCR of a 360-bp fragment of gyrA gene; sequencing of the PCR fragment from both directions
gyrAP2 TTCTTCACTCGCCTTAGTCAT
rpoBP1 TTTGATTCGCTCATGCCCCAT PCR of a 330-bp fragment of rpoB gene; sequencing of the PCR fragment from both directions
rpoBP2 CACAACCTTTTTATAAGGGGC
rdxAP1 TTTGAGCATGGGGCAGATT PCR of an 850-bp fragment covering the entire rdxA gene with primer rdxAP1 and rdxAP12; sequencing of the PCR fragment using all 5 primers
rdxAP2 GAAGAAATCGCTGAAATCG
rdxAP3 GTTAATGGTGGTATGCTCT
rdxAP4 CAAATTTGCATGGGCGTGA
rdxAP12 AGAGCGATTAAAACCATTCT