TABLE 1.
Oligonucleotide primers
Primer | Sequence (5′→3′) | Application |
---|---|---|
23SP1 | ACGGCGGCCGTAACTATA | PCR of a 307-bp fragment of 23S rRNA gene; sequencing of the PCR fragment from both directions |
23SP2 | ACAGGCCAGTTAGCTA | |
gyrAP1 | ATGCATGAATTAGGCCTTACT | PCR of a 360-bp fragment of gyrA gene; sequencing of the PCR fragment from both directions |
gyrAP2 | TTCTTCACTCGCCTTAGTCAT | |
rpoBP1 | TTTGATTCGCTCATGCCCCAT | PCR of a 330-bp fragment of rpoB gene; sequencing of the PCR fragment from both directions |
rpoBP2 | CACAACCTTTTTATAAGGGGC | |
rdxAP1 | TTTGAGCATGGGGCAGATT | PCR of an 850-bp fragment covering the entire rdxA gene with primer rdxAP1 and rdxAP12; sequencing of the PCR fragment using all 5 primers |
rdxAP2 | GAAGAAATCGCTGAAATCG | |
rdxAP3 | GTTAATGGTGGTATGCTCT | |
rdxAP4 | CAAATTTGCATGGGCGTGA | |
rdxAP12 | AGAGCGATTAAAACCATTCT |