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Examples of cases diagnosed via PCR and nested PCR were decisive in the clinically diagnosed new PB leprosy. The horizontal axis indicates the products of five DNA targets by agarose gel. Samples form clinically diagnosed PB leprosy could be detected by first PCR (A), first PCR with nested PCR (B), and nested PCR with one of target genes (C). M, marker; NC, negative control; PC, positive control; P1–P9, clinical samples.
