Table 1.
The overall positive rate of PCR and nested PCR using five different gene targets of M. leprae according to the type of skin specimens.
| Clinical sample | Bacillary index | No. of samples | Positive 16SrRNA (%) | Positive RLEP (%) | Positive FolP (%) | Positive RpoB (%) | Positive GyrA (%) | Positive Nested PCR FolP (%) | Positive Nested PCR RpoB (%) | Positive Nested PCR GyrA (%) | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MB (>5 skin lesions) | 2–5 | 202 | 162 (80) | 170 (84) | 166 (82) | 162 (80) | 166 (82) | 190 (94) | 194 (96) | 194 (96) | |
| PB | 1–5 skin lesions | 0–1 | 50 | 2 (4) | 4 (8) | 8 (15.4) | 7 (14) | 10 (19.2) | 40 (80) | 43 (82.7) | 48 (92.3) |
| Pure neuritic leprosy | 0–1 | 48 | 1 (2) | 3 (6.3) | 4 (8.3) | 3 (6.3) | 7 (14.6) | 40 (83.3) | 41 (85.4) | 43 (89.6) | |