Table 4.

Comparison of the results of molecular and ELISPOT assays using clinical samples of clinically diagnosed new PB leprosy patients and healthy donor.

Method	Gene or antigen (loci)		Positive value		p-value
PCR vs. nested PCR	PCR	Nested PCR	PCR	Nested PCR
	FolP (Outer primers)	FolP (Inner primers)	12 (12.2%)	80 (81.6%)	0.0001
	RpoB (Outer primers)	RpoB (Inner primers)	10 (10.2%)	84 (85.7%)	0.0001
	GyrA (Outer primers)	GyrA (Inner primers)	17 (17.3%)	91 (92.9%)	0.0001
	At least one gene is positive	At least one gene is positive	17 (17.3%)	91 (92.9%)	0.0001
PCR vs. ELISPOT	PCR	ELISPOT	PCR	ELISPOT
	At least one gene is positive	At least one antigen is positive	17 (17.3%)	90 (91.8%)	0.0001
Nested PCR vs. ELISPOT	nested PCR	ELISPOT	nested PCR	ELISPOT
	At least one gene is positive	At least one antigen is positive	91 (92.9%)	90 (91.8%)	1.0000