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Kinetic constants for recombinant dGK with nucleoside substrates using different phosphate donorsa
| Substrate | Phosphate donor | Apparent Km (μM)b | Apparent Vmax (nmol/mg/min)b | Efficiency (Vmax/Km) |
|---|---|---|---|---|
| dGuo | ATP | 2.8 ± 0.5 | 30 ± 5.0 | 12 |
| UTP | 3.7 ± 0.5 | 88 ± 1.5 | 23.7 | |
| CdA | ATP | 62 ± 1.0 | 518 ± 1.5 | 8.3 |
| UTP | 8 ± 0.5 | 83 ± 1.3 | 10.3 |
The values were determined by the radiochemical method using 5 mM ATP or 5 mM UTP as the phosphate donor.
Values and means and standard deviations.
