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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2022 Mar 23;442:115991. doi: 10.1016/j.taap.2022.115991

Fig. 8. 6:2 FTS and metFOSA induces liver lipid accumulation.

Fig. 8.

Human hepatocytes were treated with HFPO-DA, 6:2 FTS, FOSA, metFOSA, and etFOSA at 0.25-25 μM for 72 hours to induce liver lipid accumulation. 1:2 Palmitate and Oleate (0.5 mM, P/O) was included as positive control for lipid accumulation. Representative fluorescent images at 25 μM (A-G) were taken using an EVOS® FL Auto Cell Imaging System. Nile Red fluorescence was measured (excitation 485 nm/emission 535 nm) and normalized to DAPI fluorescence (excitation 358 nm/emission 461 nm). Fold change (H) was calculated compared to the DMSO treated cells. Calculations were done using an ANOVA followed by Dunnett’s test and * indicates P < 0.05. All values are means ± SEM; N = 3-4.