Fig. 2. The feasibility of sensing system based on ARNs (dGP4A and dTP4A). The luminescence intensity of the samples (a) red: HP1 + HP2 + aptamer/T-DNA + dGP4A + dTP4A + AFB1; (b) blue: HP2 + aptamer/T-DNA + dGP4A + dTP4A + polymerase + AFB1; (c) green: HP1 + aptamer/T-DNA + dGP4A + dTP4A + polymerase + AFB1; (d) black: HP1 + HP2 + aptamer/T-DNA + dGP4A + dTP4A + polymerase; (e) yellow: HP1 + HP2 + aptamer/T-DNA + dGP4A + dTP4A + polymerase + AFB1; the concentrations of the reaction are list below: HP1 (100 nM), HP2 (100 nM), aptamer/T-DNA (100 nM), Klenow polymerase exo− (5 U), AFB1 (30 nM), dGP4A (20 μM), dTP4A (20 μM).
