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. 2022 Apr 25;22:212. doi: 10.1186/s12870-022-03598-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — ND-FISH analysis of root-tip metaphase cells of four rye plants. a The two 1RS arms in a plant PI 315,959–4 with different signal intensities of Oligo-pSc119.2–1. b The two 1RS arms in plant PI 315,959–7 with different signal intensities of Oligo-pTa71A-2. c The two 1RS arms in plant PI 315,959–8 with different signal intensities of Oligo-pSc200. d The two 1RS arms in plant PI 315,959–5 with different signal intensities of Oligo-pSc250. The arrows indicate 1RS arms. Scale bar: 10 μm