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. 2022 Jan 28;43(3):217–230. doi: 10.1093/carcin/bgac008

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Figure 2. — The combination of MLT and Andro results in synergistic anticancer effects in CRC cells. (A) Results of CCK-8 assay comparing cell viability following treatment with MLT, Andro and their combination for 48 h in CRC cell lines. (B) Isobologram analysis of CI values based on the results of CCK-8 assay to determine synergistic effects of MLT and Andro in CRC cell lines. (C) Representative results of colony formation assay following treatment with MLT, Andro and their combination for 48 h in CRC cell lines. Colony count analysis is shown in the right panel. (D) Representative results of FACS analysis using the Muse Caspase-3/7 Kit after treatment on CRC cells by MLT, Andro and their combination for 48 h. Apoptosis rate analysis is shown in the right panel. Statistical significance: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.