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. 2022 Apr 8;13:856186. doi: 10.3389/fimmu.2022.856186

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Copyright © 2022 Zhu, Huang, Liu, Peng, Xue, Feng, Huang, Chen, Lai, Ji, Wang and Yuan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adverse Effects of LUCTA1 on HCC in vitro. (A) ENCORI server analyzed the expression of the LUCAT1 gene in HCC. (B) Kaplan–Meier curve of the expression level of LUCAT1 on HCC patients using ENCORI. (C) qRT-PCR analysis of LUCAT1 mRNA levels in HCC tissues and corresponding adjacent tissues (n=37). (D) The efficiency of knockdown of LUCAT1 expression in MHCC97H cells was verified by qRT-PCR. (E) After LUCAT1 silencing, the cell viability of MHCC97H was significantly inhibited by the CCK- 8 assay. (F) Compared with the control group, the proliferation rate of MHCC97H cells was significantly inhibited after LUCAT1 silencing by EdU staining. (G) Transwell experiments showed that the migratory ability of MHCC97H was inhibited after LCUAT1 silencing. (H) Wound healing array showed that LUCAT1-downregulated MHCC97H cells exhibited significantly delayed wound healing compared with controls. Scale bar: 50μm, *p <0.05, **p <0.01, ***p <0.001.