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. 2022 Apr 7;10:731831. doi: 10.3389/fcell.2022.731831

FIGURE 3.

FIGURE 3

Temozolomide (TMZ), a neurogenic inhibitor, abolishes naringin effects on NSPC differentiation and antidepressant behavior. (A) Diagram showing the protocols of BrdU incorporation, Ng treatment and TMZ administration in CORT-induced depression mouse model. (B) Representative immunofluorescent imaging results showing the newly proliferative cells (MCM2+/BrdU+). DAPI was used for nuclear labeling. Scale bar 200 μm (C) Statistic analysis of MCM2+ cells in the DG region (n = 4–5 per group, unpaired student-t test, ***p < 0.001). (D) Statistic analysis of BrdU+ cells in the DG region. (E) Representative fluorescent imaging showing the newborn immature neurons (BrdU+/DCX+). DAPI was used for nuclear labeling. Scale bar 100 μm. White triangle points for newborn immature neurons with dendritic fiber; empty triangle points for newborn immature neurons without dendritic fiber. (F) Statistic analysis of DCX+ cells in the DG region. (G) Statistic analysis of immature neurons (BrdU+/DCX+) in the DG region. (H) Statistic analysis of DCX+ dendritic fiber density in the molecular layer (ML) subregion (n = 5 per group, unpaired student-t test, *p < 0.05). (I) Statistical analysis of BrdU+/DCX+ newborn immature neurons (with dendrite/without dendrite). (J) Statistical analysis of immobility time in FST. N = 4–5 per group. *p < 0.05, **p < 0.01, ***p < 0.001.