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. 2022 Apr 7;10:731831. doi: 10.3389/fcell.2022.731831

FIGURE 4.

FIGURE 4

CREB mediates the neurogenic and antidepressant effects of naringin on the CORT-induced depression mice. (A) Western blot bands showing the expression of mature and proBDNF, p-TrkB, and TrkB as well as the p-CREB (Ser133) and CREB protein in hippocampus of the CORT-induced depression mice. β-actin was settled as loading control. (B) Statistical analysis of mature BDNF/proBDNF, p-TrkB/TrkB level and pCREB/CREB level in the CORT-induced depression mice with or without Ng treatment. (C) Diagram showing the specific target of different inhibitors. (D) Diagram showing the experimental procedure for treating inhibitor Cyc-B and 666-15, respectively, in Ng-treated mice. (E) Representative fluorescent imaging showing newborn immature neurons (BrdU+/DCX+) in the hippocampal DG region. NeuN was used for labeling mature neurons. Scale bar = 100 μm. (F) Statistical analysis on the of BrdU+/DCX+ cells between vehicle and inhibitor treatment. (G) Statistical analysis of immobility time in FST between vehicle and inhibitor treatment. n = 5–6 per group. *p < 0.05, **p < 0.01, ***p < 0.001.