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. 2001 Mar;45(3):768–775. doi: 10.1128/AAC.45.3.768-775.2001

FIG. 4.

FIG. 4

Effect of various concentrations of substrates, UDP–MurNAc-pp (A and B) and UDP-GlcNAc (C and D) on the synthesis of peptidoglycan (A and C) or on the product captured in the SPA (B and D). In panels A and B the concentration of UDP-GlcNAc was 2.5 μM, and in panels C and D the concentration of UDP–MurNAc-pp was 100 μM. In panels C and D, for concentrations of UDP-GlcNAc of 0.25 to 5 μM a specific activity of 8 Ci/mmol was used, and for 10 to 25 μM a specific activity of 1.5 Ci/mmol was used. Because of the varying specific activity, the paper chromatography results are plotted as picomoles of GlcNAc incorporated, and for the SPA a correction was made for the two higher concentrations of UDP-GlcNAc so that the cpm are what would be expected if a specific activity of 8 Ci/mmol was used.