The stability of the seven flavonoids in rat plasma (n = 3).
| Compounds spiked concentration (ng mL−1) | Short-tern stability (room temperature for 8 h) | Long-tern stability (−20 °C for 21 d) | Free-thaw stability (3 free thaw cycles) | Post-preparation stability (room temperature for 4 h) | ||||
|---|---|---|---|---|---|---|---|---|
| Measured concentration (ng mL−1) | Accuracy (%) | Measured concentration (ng mL−1) | Accuracy (%) | Measured concentration (ng mL−1) | Accuracy (%) | Measured concentration (ng mL−1) | Accuracy (%) | |
| Linarin | ||||||||
| 6.85 | 7.07 ± 1.76 | 3.21 | 7.10 ± 17.48 | 3.65 | 7.02 ± 0.73 | 2.48 | 7.22 ± 1.36 | 5.40 |
| 723.73 | 755.28 ± 10.77 | 4.36 | 766.72 ± 17.29 | 5.94 | 754.99 ± 16.54 | 4.32 | 740.38 ± 7.66 | 2.30 |
| 2861.18 | 2930.71 ± 17.78 | 2.43 | 3056.88 ± 21.55 | 6.84 | 2962.18 ± 18.46 | 3.53 | 2986.21 ± 12.59 | 4.37 |
| Rutin | ||||||||
| 6.05 | 6.36 ± 6.81 | 5.12 | 6.31 ± 5.88 | 4.30 | 6.2 ± 1.88 | 2.48 | 6.26 ± 0.48 | 3.47 |
| 102.57 | 108.48 ± 7.41 | 5.76 | 107.37 ± 9.98 | 4.68 | 110.30 ± 8.24 | 7.54 | 106.61 ± 13.32 | 3.94 |
| 418.53 | 426.82 ± 18.15 | 1.98 | 429.20 ± 19.79 | 2.55 | 437.7 ± 10.02 | 4.58 | 440.50 ± 16.46 | 5.25 |
| Luteolin | ||||||||
| 6.32 | 6.76 ± 0.18 | 6.96 | 6.7 ± 0.76 | 6.01 | 6.60 ± 0.18 | 4.43 | 6.50 ± 0.16 | 2.85 |
| 62.17 | 64.88 ± 0.92 | 4.36 | 67.34 ± 3.63 | 8.32 | 66.03 ± 2.64 | 6.21 | 64.29 ± 0.37 | 3.41 |
| 247.58 | 260.55 ± 0.49 | 5.24 | 261.02 ± 4.65 | 5.43 | 258.35 ± 4.86 | 4.35 | 253.42 ± 2.77 | 2.36 |
| Quercetin | ||||||||
| 2.64 | 2.80 ± 1.62 | 6.06 | 2.72 ± 0.32 | 3.03 | 2.78 ± 0.89 | 5.30 | 2.74 ± 0.32 | 3.79 |
| 16.38 | 17.12 ± 1.22 | 4.52 | 17.27 ± 2.69 | 5.43 | 17.48 ± 1.19 | 6.72 | 16.78 ± 1.77 | 2.44 |
| 64.48 | 66.98 ± 7.47 | 3.88 | 68.36 ± 3.68 | 6.02 | 67.46 ± 2.42 | 4.62 | 66.99 ± 4.58 | 3.89 |
| Apigenin | ||||||||
| 7.08 | 7.17 ± 1.91 | 1.27 | 7.58 ± 0.51 | 7.06 | 7.34 ± 0.32 | 3.67 | 7.33 ± 0.16 | 3.53 |
| 116.93 | 123.78 ± 2.47 | 5.86 | 124.51 ± 4.02 | 6.48 | 124.68 ± 6.33 | 6.63 | 122.61 ± 7.12 | 4.86 |
| 468.54 | 490.89 ± 7.31 | 4.77 | 484.98 ± 5.09 | 3.51 | 494.36 ± 4.99 | 5.51 | 485.92 ± 3.94 | 3.71 |
| Acacetin | ||||||||
| 4.62 | 4.69 ± 0.36 | 1.52 | 4.81 ± 0.27 | 4.11 | 4.81 ± 0.22 | 4.11 | 4.78 ± 0.18 | 3.46 |
| 26.17 | 27.31 ± 5.34 | 4.36 | 26.53 ± 3.21 | 1.38 | 26.99 ± 4.36 | 3.13 | 27.25 ± 3.96 | 4.13 |
| 102.14 | 107.02 ± 3.38 | 4.78 | 105.68 ± 9.65 | 3.46 | 108.48 ± 6.51 | 6.21 | 107.29 ± 6.25 | 5.04 |
| Acteoside | ||||||||
| 2.03 | 2.11 ± 0.13 | 3.94 | 2.07 ± 0.12 | 1.97 | 2.1 ± 0.14 | 3.45 | 2.16 ± 0.35 | 6.40 |
| 519.32 | 548.82 ± 2.92 | 5.68 | 540.92 ± 9.44 | 4.16 | 544.87 ± 4.54 | 4.92 | 542.64 ± 4.56 | 4.49 |
| 2027.29 | 2150.14 ± 11.92 | 6.06 | 2167.98 ± 18.52 | 6.94 | 2132.91 ± 11.91 | 5.21 | 2140.01 ± 9.32 | 5.56 |