TABLE 3.
SSCP analysis of P. carinii DHPS mutations in 41 clinical isolates
| No. of isolates | DHPS codons 55 and 57a determined by:
|
|
|---|---|---|
| SSCP | Direct sequencing | |
| 24b | T-55, P-57 | T-55, P-57 (9)c |
| 2 | A-55, P-57 | A-55, P-57 |
| 3 | A-55, S-57 | A-55, S-57 |
| 2 | A/T-55, P-57 | A/T-55, P-57 |
| 3 | A/T-55, P-57 | T-55, P-57 |
| 2 | A/T-55, S/P-57 | A/T-55, S/P-57 |
| 2 | A/T-55, S/P-57 | A/T-55, P-57 |
| 3 | A/T-55, S/P-57 | T-55, P-57 |
a
Amino acids that differ from those in the wild-type sequence are underlined; the genotype A/T-55, S/P-57 represents a mixture of wild-type (T-55, P-57) and double mutant (A-55, S-57) sequences.
b
Including two isolates containing a synonymous change at codon 51 or 73, respectively.
c
Only nine isolates were examined by direct sequencing.