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. 2022 Apr 18;11(1):1135–1144. doi: 10.1080/22221751.2022.2059403

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — SARS-CoV-2 pseudovirus enters the host cells through CD147-mediated endocytosis. (A–C) Left, the knockdown or knockout of CD147 in Vero E6 and Huh-7 cells was detected by RT-PCR (**P < 0.01, ***P < 0.001). Right, SARS-CoV-2 pseudovirus infection in different cells was performed by the luciferase reporter assay (*P < 0.05, ***P < 0.001). (D, E) Left, Rab5a knockdown in Vero E6 and Huh-7 cells was detected by RT-PCR (*P < 0.05, ***P < 0.001). Right, SARS-CoV-2 pseudovirus infection in siRab5a cells and the control cells was performed by luciferase reporter assay (*P < 0.05, **P < 0.01). (F) The co-localization between Rab5a (red) and CD147 (white) was analyzed in Vero E6 cells by immunofluorescence staining. Scale bars, 25 μm. (G, H) Relative Rab5a mRNA level was detected by RT-PCR in the control and Vero E6-CD147KO or Huh-7-siCD147 cells (**P < 0.01, ***P < 0.001). (I, J) The co-localization of spike protein (green), Rab5a (red), and CD147 (white) were analyzed in Vero E6 cells and CD147 silencing cells by multicolour immunofluorescence staining. Scale bars, 25 μm.