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. 2022 Apr 18;11(1):1135–1144. doi: 10.1080/22221751.2022.2059403

Figure 3.

Figure 3.

SARS-CoV-2 pseudovirus enters the host cells through spike protein-CD147 in an Arf6-dependent manner. (A) Vero E6 cells were pretreated with six inhibitors, respectively, for 24 h, and incubated with SARS-CoV-2 pseudovirus. The SARS-CoV-2 pseudovirus infection in the different groups was detected by the luciferase reporter assay (*P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant). (B) Vero E6-CD147KO cells were pretreated with four inhibitors (MCD, filipin III, cyto D, and nocodazole) respectively for 24 h, and incubated with SARS-CoV-2 pseudovirus. The SARS-CoV-2 pseudovirus infection in the different groups was detected by the luciferase reporter assay (*P < 0.05, **P < 0.01). (C-E) Left, the knockdown of Arf6 in Vero E6, Huh-7, and Vero E6-CD147KO cells was detected by RT-PCR (***P < 0.001). Right, SARS-CoV-2 pseudovirus infection in different cells was performed by the luciferase reporter assay (**P < 0.01, ns, not significant).