Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 5;13:860170. doi: 10.3389/fpls.2022.860170

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Guo, Chen, Gao and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

EG1 interacts with OsGABA-T. (A) Interactions between EG1 with OsGABA-T in the DUAL membrane yeast 2 hybrid system. Yeast NMY32 cells cotransformed with bait and prey vectors were grown on QDO (SD/–Ade/–His/–Leu/–Trp) medium. The combination of pTSU2-APP and pNubG-Fe65 was used as a positive control, while the combination of pTSU2-APP and pPR3N was used as the negative control. (B) Interactions between EG1 with OsGABA-T in Nicotiana benthamiana. pGR106 carried EG1-EGFP and OsGABA-T-HA were coexpressed in Nicotiana benthamiana; 48 h after infiltration, immunoprecipitates obtained from whole-cell extracts using anti-GFP trap beads were analyzed by immunoblotting with anti-HA and anti-GFP antibodies. This experiment was repeated three times with similar results.