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. 2022 Apr 5;13:860170. doi: 10.3389/fpls.2022.860170

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Copyright © 2022 Guo, Chen, Gao and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OsGABA-T suppresses allergic necrosis induced by EG1. (A) Transient expression of EG1 and OsGABA-T in Nicotiana benthamiana. Transient expression was operated in Nicotiana benthamiana leaves injected Agrobacterium carrying the indicated genes, pGR106, eGFP, EG1, and OsGABA-T. eGFP and EG1 were coexpressed on the left of the leaf, OsGABA-T and EG1 were coexpressed on the right. Experiments were repeated three times at least with similar results. (B) ROS detection of EG1 and OsGABA-T. The transient expression of Nicotiana benthamiana leaves inoculated with eGFP and EG1, and OsGABA-T and EG1 were dyed with 5 mg/mL 3,3-diaminobenzidine (DAB). Transient expression was assessed in Nicotiana benthamiana leaves from 4-week-old plants 5 days after agroinfiltration, and pGR106 empty vector was used as a control. Experiments were repeated three times at least with similar results.