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. 2022 Apr 5;13:860170. doi: 10.3389/fpls.2022.860170

FIGURE 8.

FIGURE 8

Knockout of OsGABA-T reduces rice disease resistance to Rhizoctonia solani. (A) Sequence of gRNA target of rice. Red color represents the target sequence. (B) OsGABA-T transcription levels in wild-type ZH11 and OsGABA-T knocked out rice lines, Os-24 and Os-31. Error bars represent ± SD of at least three independent tests. Lower-case letters indicate statistically significant differences (P < 0.05). (C) Inoculation of wild-type ZH11 and OsGABA-T knocked out lines Os-24 and Os-31 with Rhizoctonia solani AG1-IA. Images were taken 72 h after inoculation. (D) Lesion length of infected rice leaves. Lesion length was calculated from three independent biological replicates. And the red arrows indicated lesion length. Error bars represent ± SD of at least six leaves each. Lower-case letters indicate statistically significant differences (P < 0.05). (E) Quantitative PCR was performed for quantification of relative fungal growth on inoculated leaves 2(Ct_OsUbiq–Ct_RsDNA). Error bars represent ± SD of 6 independent tests. Lower-case letters indicate statistically significant differences (P < 0.05).