FIGURE 1.

Single-cell sequencing analysis of the target cells enriched by fluorescence-activated cell sorting (FACS). (a) Cell surface markers or fluorescent reporter proteins can mark a group of cells expressing the marker genes at a given time (“Snapshot”). (b) In vivo lineage-tracing system can mark a specific group of cells descended from a specific type of cell, therefore including a “temporal factor”. Single-cell sequencing analyses can reveal the heterogeneity of the lineage-marked cells with inference to precursor-product relationships.