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. 2022 Mar 21;154(9):e202112994. doi: 10.1085/jgp.202112994

Table A4.

Parameter values used for assessing effect of TnC on Ca2+–EGTA equilibrium

Parameter Value Comments
EGTA 50,000 μM
k 1+ 1.67 M−1 s−1 From Kd of 0.18 μM (Smith et al., 1984) and k1−
k 1− 0.31 s−1 Smith et al. (1984)
Tn 100 μM 2 Ca2+ binding sites/molecule (i.e., binding sites, 200 μM)
k 2+ 240 M−1 s−1 Baylor et al. (1983) for rabbit TnC, adjusted from 16°C to 25°C assuming a Q10 of 2.0
k 2− 2,900 s−1
k 3+ 4.7 M−1 s−1
k3- 165 s−1