Figure 5.

Circ_0007059 knockdown inhibits H₂O₂-induced cardiomyocyte inflammation. Cardiomyocytes were infected with lentiviral-si-circ_0007059/siNC for 1 d and subsequently exposed to H₂O₂ (0.1 mM) for 1 d. (a) WB quantification for inflammatory cytokine expression in cardiomyocytes. (B, C, D) ELISA for the quantification of inflammatory cytokines released into the cardiomyocyte medium. (e) DCFH-DA staining was performed to evaluate the intra-cellular ROS production. (f) Activity of anti-oxidative enzyme SOD was determined using SOD assay kits. (g) WB detection of the Nrf2 and HO-1 protein levels in cardiomyocytes. *P < 0.05 and **P < 0.01 versus the CG; ^#P < 0.05 versus the H₂O₂ group.