Figure 5.

USP19 negatively regulates antiviral signaling by targeting TBK1. (A-F) HEK293T cells were transfected with different concentrations of USP19 plasmids and DDX58 (A and B), MAVS (C and D) or TBK1 (E and F) plasmids. Dual-luciferase reporter assays were performed 24 h after transfection. Exogenous USP19 protein levels were analyzed via immunoblotting. (G) HEK293T cells were transfected with MYC-DDX58, MYC-MAVS, MYC-TBK1, MYC-IRF3, MYC-IFIH1, His-CGAS, His-STING1, His-TBK1, and Flag-USP19 expression plasmids for 24 h before the lysates were subjected to immunoprecipitation with an anti-MYC or His antibody, and the results were analyzed via immunoblotting. The data are representative of three independent experiments. Error bars show the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 using Student’s t test.