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. 2021 Aug 26;18(4):891–908. doi: 10.1080/15548627.2021.1963155

Figure 6.

Figure 6.

Knockdown of USP19 promotes cellular antiviral response in human PBMCs. (A-E) PBMCs were transfected with control or USP19 siRNAs for 48 h, with or without pretreatment with VER-155,008 (5 μM) for 30 min. USP19 expression was analyzed via qPCR (A) and immunoblotting (B). qPCR analysis of IFNB1 expression 12 h after VSV infection (MOI = 1) (C). ELISA analysis of IFNB production in PBMC supernatants 12 h after VSV infection (MOI = 1) (D). qPCR analysis of VSV replication 12 h after VSV infection (MOI = 1) (E). (F and G) PBMCs were transfected with control or USP19 siRNAs for 48 h, VSV infection (MOI = 1) was performed at the indicated times, and the indicated proteins were analyzed via immunoblotting. The data are representative of three independent experiments. Error bars show the means ± SD. *p < 0.05, **p < 0.01 using Student’s t test.