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. 2021 Aug 2;18(4):816–828. doi: 10.1080/15548627.2021.1959086

Figure 4.

Figure 4.

STING1 serves as an upstream molecule of EIF2AK3-EIF2A signaling to induce autophagy. (A) Stable STING1 KD PK15 cells were verified by western blot. TUBB3 was used as a loading control. (B) qPCR-measured copy numbers of FMDV in FMDV-infected wild-type and STING1 KD cells (MOI = 1, 3 hpi). The data show the mean ± SD; n = 3; ***P < 0.001. (C) Wild-type and STING1 KD cells were infected with FMDV (MOI = 1), and virus titers were measured by TCID50 (3 hpi). The data show the mean ± SD; n = 3; ****P < 0.0001. (D) Western blot analysis of FMDV VP1 protein in FMDV-infected wild-type and STING1 KD cells (MOI = 1, 3 hpi). (E) FMDV-infected (MOI = 1) and uninfected STING1 KD cells were analyzed by immunofluorescence using anti-MAP1LC3B antibodies at 3 hpi. The fluorescence signals were visualized by confocal immunofluorescence microscopy. Scale bar: 10 μm. (F) Western blot analysis of MAP1LC3B, phosphorylated EIF2AK3 and EIF2A levels in FMDV-infected (MOI = 1) and uninfected STING1 KD cells (3 hpi). TUBB3 was used as a loading control.