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. 2021 Jun 8;18(3):518–539. doi: 10.1080/15548627.2021.1936356

Figure 2.

Figure 2.

Effect of G. cambogia extract-induced RPS6KA1 and STAT3 inhibition on CEBPB regulation in MDI-induced 3T3-L1 preadipocytes during differentiation. (A) Effect of G. cambogia extract (300 μg/ml) on CEBPB (LAP* and LAP) and CEBPD expression in MDI-induced 3T3-L1 preadipocytes for the indicated times (n = 4 per group). MDI: MDI-treated cells. Ex.: Exposure. *p < 0.05 and **p < 0.01 vs. each control. n.s.: not significant. (B) Effect of G. cambogia extract (0–300 μg/ml) on CEBPB (LAP* and LAP) and CEBPD expression in MDI-induced 3T3-L1 preadipocytes after 48 h (n = 4 per group). Con: MDI-untreated cells. **p < 0.01 vs. Con, ##p < 0.01 vs. MDI. (C) Effect of Rps6ka1 and Stat3 knockdown on CEBPB (LAP* and LAP) and CEBPD expression in 3T3-L1 differentiated cells. (D) Effect of FMK (3 μM) and stattic (5 μM) on CEBPB (LAP* and LAP) and CEBPD expression in MDI-induced 3T3-L1 preadipocytes for the indicated duration (n = 4 per group). *p < 0.05 and **p < 0.01 vs. each control. (E) Effect of G. cambogia extract, FMK (3 μM) and stattic (5 μM) on CREB phosphorylation in MDI-induced 3T3-L1 preadipocytes after 60 min (n = 4 per group). **p < 0.01 vs. Con, ##p < 0.01 vs. MDI. (F) Effect of G. cambogia extract (300 μg/ml) on the Cebpb transcript level in MDI-induced 3T3-L1 preadipocytes for the indicated times (n = 9 per group). **p < 0.01 vs. each control. (G) Effect of G. cambogia extract (300 μg/ml) on the CEBPB protein half-life in MDI-induced 3T3-L1 preadipocytes. After treatment of G. cambogia extract in cells for 42 h, translation inhibitor cycloheximide (CHX, 1.5 μg/ml) was coincubated with the indicated times (n = 4 per group). The data are the mean ± S.D.