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. 2021 Jun 8;18(3):518–539. doi: 10.1080/15548627.2021.1936356

Figure 7.

Figure 7.

Effect of G. cambogia extract on KLF3 and CTBP2 interaction to regulate adipogenic factors in 3T3-L1 preadipocytes during differentiation. (A) Effect of G. cambogia extract (0–300 μg/ml) on CTBP2 expression in 3T3-L1 differentiated cells for 72 h (n = 4 per group). n.s.: not significant. (B) Effect of FMK (3 μM) and stattic (5 μM) on CTBP2 expression in 3T3-L1 differentiated cells for the indicated times (n = 4 per group). (C) Physical interaction of KLF3 and CTBP2 in 3T3-L1 differentiated cells treated with G. cambogia extract (Ga, 300 μg/ml), FMK (3 μM) and stattic (5 μM) for 72 h. Coimmunoprecipitation (IP) was used to analyze the interaction of KLF3 and CTBP2. The lysates were immunoprecipitated with anti-KLF3 and anti-IgG antibodies, and the precipitates were analyzed by western blotting using antibody against CTBP2 (n = 4 per group). (D) Fluorescence photographs (left) and quantification data (right) of KLF3 and CTBP2 in 3T3-L1 differentiated cells treated with G. cambogia extract (300 μg/ml), FMK (3 μM) and stattic (5 μM) for 72 h. The fluorescence intensity and colocalization of TRITC (i.e., KLF3) and FITC (i.e., CTBP2) were quantified using ImageJ software. Colocalization was analyzed using the Pearson correlation coefficient. Scale bars: 5 μm. (n = 6 per group). **p < 0.01 vs. Con, n.s.: not significant. (E) Effect of G. cambogia extract (300 μg/ml) on the transcript levels of Cebpa and Pparg in 3T3-L1 differentiated cells for the indicated times (n = 9 per group). **p < 0.01 vs. each control. The data are the mean ± S.D.