Effect of G. cambogia extract on HFD-induced obesity in mice. (A) Effect of G. cambogia extract on high-fat diet (HFD)-induced body weight, and (B) weight gain, caloric intake and energy efficiency rate (EER) (n = 8 per group). Body weight and food intake were monitored twice per week for 8 weeks. Normal diet (ND)-fed mice served as a negative control for high-fat accumulation, and orlistat (20 mg/kg) was used as a positive control for antiobesity effects. (C and D) Effect of G. cambogia extract on HFD-induced adipose fat tissue mass and (E) quantification data (n = 8 per group). eWAT: epididymal white adipose tissue; mWAT: mesenteric white adipose tissue; rWAT: retroperitoneal white adipose tissue; iWAT: inguinal subcutaneous white adipose tissue; BAT: brown adipose tissue. (F) Effect of G. cambogia extract on adipocyte size in HFD-induced eWAT and iWAT. H&E staining of eWAT and iWAT was performed for histological analysis, and adipocyte size was estimated using ImageJ software Adiposoft. Scale bars: 50 μm. (G) Serum analysis of triglyceride and total cholesterol levels (n = 7 per group). *p < 0.05, **p < 0.01 vs. ND-fed mice, #p < 0.05 and ##p < 0.01 vs. HFD-fed mice. n.s.: not significant. The data are the mean ± S.D.